recombinant virus造句
例句与造句
- Two recombinant viruses : vac - ha2 - gfp and delac9 - ac - ha2 - gfp was expected to give the answer
构建了表达haz与acg两个同源基因的重组病毒vac - haz一g印。 - After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis
之后利用pcr分析是否获得重组angiostatin的杆状病毒。 - The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus
另外，通过共转染和筛选纯化获得了携带appa基因的重组家蚕杆状病毒。 - Sds - page analysis of pcr product of the recombinant virus showed with the amplified fregment was in conformity with the expected size , suggesting correct recombination and cloning of the ge gene
对重组病毒进行pcr鉴定，电泳结果显示扩增片段的大小与预期值相符， ge基因重组、克隆正确。 - The recombinant virus adhuctla4 - ig prepared in this study efliciently inltcted l - o2 " cells , and the infected cells expressed a - nd excreted soluble rccolllbina11t protein huctla4 - ig
该重组病毒在体外能有效感染正常肝细胞株l - o2 ，受感染细胞能表达、分泌可溶性的重组融合蛋白huctla4 - ig ； 2 - It's difficult to find recombinant virus in a sentence. 用recombinant virus造句挺难的
- Furtherly expression phase analysis showed that orf2 appeared in different form during infection . to reveal the function of haorf2 , vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated
构建了ha2与绿色荧光蛋白融合表达的重组病毒vha - orf2 - gfp ，对该重组病毒感染细胞后，融合篡硕士学位论文mast卜r ’ s ’ r拼ifs - The polyhedrin gene sequence in recombinant virus bmpak - hbmp can be more benefit to enhancing the expression of the hbsag ( pres2 + s ) from atg initial codon at the 5 " ends of pres2 gene
另一方面， elisa检测表明， presz抗原性提高率约是hbsag的14倍，提示bmpak hb帅中多角体基因部分碱基序列的存在更有利于从presz前的atg开始表达中蛋白全基因（ presz s ） 。 - Then bm cell line was co - transfected with parental bm - npv dna and the transfer plasmid pvl - nk , the recombinant virus was then obtained subsequently with routine procedure . nattokinase was expressed in silkworm larva by injection with the recombinant virus
将nk基因克隆至转移载体pvl - 1393中后用常规方法获得重组病毒，感染家蚕后纳豆激酶获得正确表达。 - To study the localization of the peptide , we also generated recombinant viruses containing the ctl - egfp fusion gene , following observation of infected larvae by such viruses will provide insight into the function of baculoviral conotoxinlike genes
为了研究毒素在虫体中的定位，构建了类蜗牛毒素和增强的绿荧光蛋白融合的重组病毒racbacctlgfp2和rhabacctlgfp ，为下一步研究奠定了基础。 - Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31 , ' 30 , 28 and 25 kd in the western blotting profiles , due to the two atg initial codons at the 5 " ends of both pres2 and s gene
Western杂交结果表明，感染bmpak hbmp和bmpak hbm的家蚕蛹血淋巴中均有4种不同分子量的蛋白质和鼠抗人的一抗发生免疫反应，大小约分别为31kd 、 30kd 、 zskd 、 25kd 。 - A deletion recombinant virus ( hasnpva132 ) of hal32 was generated by homologous recombination in e . coli . electron microscope pictures revealed the deletion virus could replicate in hzaml cells , which indicates hal32 is not essential for the replication of hasnpv
通过在大肠杆菌内同源重组构建得到orf132的缺失重组病毒（ hasnpv凸132 ） ，转染成功后的电镜切片表明， hasnpv凸132能够在hzami细胞内正常复制、增殖。 - The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol . to purify the recombinant virus , we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
构建重组病毒：用已经构建好的angiostatin杆状病毒转移载体pbluebachiszb和病毒dna共同转染sfg细胞，通过蚀斑实验筛选出纯的重组斑点并扩增产生p二病毒贮存液。 - Haorf2 protein was found to localize in cell nucleus with this recombinant virus , and the fusion protein appeared to assemble into virions normally . according to the study on the interaction between haorf2 protein and actin , haorf2 seemed to colocalize with actin during infection
S蛋白在hzami细胞进行了亚细胞定位分析，以及与actin共定位的分析，结果表明， haz与gfp融合后，到感染晚期定位于细胞核中，这与haz结构蛋白的特性相符。 - Trying to understand p78 / 83 function , we first constructed recombinant virus vac - orf9 - gfp with bac - to - bac system , within which acorf9 was fused with egfp . sf21 cells were infected with the recombinant virus and laser confocal microscopy was used to determine the subcellular location of p78 / 83
通过bac - to - bac系统构建ac9与egfp融合表达的重组病毒vac - orf9 - gfp ，对重组病毒表达的融合蛋白进行了亚细胞定位分析，以及与actin共定位的分析。 - The purpose of this study is to construct a recombinant pseudorabies virus expressing ha gene of swine influenza virus and to develop specific diagnosis for differentiating the recombinant virus immunized animals and those naturally infected with prv or sfv
本试验的目的是以ge基因缺失的prv为载体构建可以表达sivha基因的重组病毒疫苗用于预防这两种病，同时，基于此重组病毒的特点，建立可以区分此重组病毒疫苗和prv及siv自然感染的鉴别诊断方法。
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